Assay-Prinzip
Caspase 1 Assay Kit (Colorimetric) is based on the hydrolysis of the peptide substrate Ac-YVAD-pNA (acetyl-Tyr-Val-Ala-Asp p-nitroanilide) by caspase 1, resulting in the release of the p-nitroaniline (pNA), which can be quantified using a spectrophotometer or a microtiter plate reader at 405 nm.