Anti-Cardiac Troponin T Antibody [ARC1242] (A308336)

Name: Anti-Cardiac Troponin T Antibody [ARC1242]
Brand: Antibodies.com
SKU: A308336
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1242] antibody to Cardiac Troponin T for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Cardiac Troponin T Antibody [ARC1242]

Description

Rabbit monoclonal [ARC1242] antibody to Cardiac Troponin T.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 199-298 of human Cardiac troponin T (TNNT2) (P45379).

Sequence

QKQAQTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQSIYNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1242

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

36 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Cardiac muscle troponin T, cTnT, TNNT2, TnTc, Troponin T, cardiac muscle

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-Cardiac Troponin T Antibody [ARC1242] (A308336)

Western blot analysis of extracts of Mouse skeletal muscle, using Anti-Cardiac Troponin T Antibody [ARC1242] (A308336) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Immunohistochemistry - Anti-Cardiac Troponin T Antibody [ARC1242] (A308336)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-Cardiac Troponin T Antibody [ARC1242] (A308336) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Cardiac Troponin T Antibody [ARC1242] (A308336)

Immunofluorescence analysis of rat heart using Anti-Cardiac Troponin T Antibody [ARC1242] (A308336) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Cardiac Troponin T Antibody [ARC1242] (A308336)

Immunofluorescence analysis of mouse heart using Anti-Cardiac Troponin T Antibody [ARC1242] (A308336) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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