Anti-Calpain 1 Antibody [ARC1272] (A305565)

Name: Anti-Calpain 1 Antibody [ARC1272]
Brand: Antibodies.com
SKU: A305565
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC1272] antibody to Calpain 1 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Calpain 1 Antibody [ARC1272]

Description

Rabbit monoclonal [ARC1272] antibody to Calpain 1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Calpain 1 (P07384).

Sequence

DGATRTDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLPIKDGKLVFVHSAEGNEFWSALLEKAYAKVNG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1272

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

75 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Calcium-activated neutral proteinase 1, Calcium-activated neutral proteinase 10, Calpain mu-type, Calpain-1 catalytic subunit, Calpain-1 large subunit, Calpain-10, CANP 1, CANP 10, CANPL1, CAPN1, CAPN10, Cell proliferation-inducing gene 30 protein, KIAA1845, Micromolar-calpain, muCANP

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-Calpain 1 Antibody [ARC1272] (A305565)

Western blot analysis of extracts of various cell lines, using Anti-Calpain 1 Antibody [ARC1272] (A305565) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Immunofluorescence - Anti-Calpain 1 Antibody [ARC1272] (A305565)

Immunofluorescence analysis of C6 cells using Anti-Calpain 1 Antibody [ARC1272] (A305565) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Calpain 1 Antibody [ARC1272] (A305565)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Calpain 1 Antibody [ARC1272] (A305565) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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