Rabbit polyclonal antibody to Calnexin (phospho Ser583).
Spezifität
This antibody detects endogenous levels of Calnexin only when phosphorylated at Ser583.
Anwendungen
WB, IHC, IF, ELISA
Verdünnungen
WB: 1:500-1:1000, ELISA: 1:1000
Reaktivität
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human Calnexin around the phosphorylation site of Ser583 (amino acids 543-592).
Wirt
Rabbit
Klonalität
Polyclonal
Isotyp
IgG
Konjugat
Unconjugated
Reinigung
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molekulargewicht
67kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
CALX_HUMAN, CANX, CNX, FLJ26570, Histocompatibility complex class I antigen binding protein p88, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90
Figure 1: Western Blot - Anti-Calnexin (phospho Ser583) Antibody (A94794)
Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30' using Anti-Calnexin (phospho Ser583) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-Calnexin (phospho Ser583) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunofluorescence analysis of HeLa cells using Anti-Calnexin (phospho Ser583) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.