Mouse monoclonal [CALD1/820] antibody to Caldesmon.
Spezifität
This antibody recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (12080kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This antibody recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
Anwendungen
WB, IHC-P
Verdünnungen
WB: 2-4 µg/ml, IHC-P: 0.25-0.5 µg/ml
Reaktivität
Human
Kreuzreaktivität
This antibody does not cross react with Rat.
Immunogen
Recombinant full-length human CALD1 protein.
Wirt
Mouse
Klonalität
Monoclonal
Klon
CALD1/820
Isotyp
IgG1
Leichte Ketten
kappa
Konjugat
Unconjugated
Reinigung
Protein A/G chromatography.
Konzentration
200 µg/ml
Molekulargewicht
150 kDa
Produktform
Liquid
Formulierung
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-Caldesmon Antibody [CALD1/820] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.