Anti-Brd4 (phospho Ser1083) Antikörper [ARC5019-01] (A306225)

Western blot analysis of extracts of various cell lines, using Anti-Brd4 (phospho Ser1083) Antibody [ARC5019-01] (A306225) at 1:500 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. HeLa cells were treated by nocodazole (50 ng/ml) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 300s.