Figure 1: Western Blot - Anti-BNIP3 Antibody (A304942)
Western blot analysis of mouse kidney showing detection of ~30kDa BNIP3 protein using Anti-BNIP3 Antibody (A304942) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: mouse kidney (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at room temperature. Color Development: TMB solution for 12 min at room temperature. Predicted/Observed Size: ~30kDa. Other Band(s): ~60kDa (BNIP3 complex).
Figure 2: Western Blot - Anti-BNIP3 Antibody (A304942)
Western blot analysis of human HeLa and HEK293T cell lysates showing detection of ~30kDa BNIP3 protein using Anti-BNIP3 Antibody (A304942) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: human HeLa (20 µg). Lane 3: human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at room temperature. Color Development: TMB solution for 12 min at room temperature. Predicted/Observed Size: ~30kDa.
Immunocytochemistry/Immunofluorescence analysis of human fibroblast cell line (NIH 3T3), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-BNIP3 Antibody (A304942), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BNIP3 Antibody (D) Composite.
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