Anti-Bif-1 Antikörper (A83782)

Name: Anti-Bif-1 Antibody
Brand: Antibodies.com
SKU: A83782
Price: 480.0 USD
Availability: InStock

Dieses Produkt ist identisch mit Novus (NB100-820). Entdecken Sie mehr für weniger.

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$480

Polyklonal Ziege Antikörper gegen Bif-1 für ELISA, WB, IHC und Flow Cytometry.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 3-5 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Produktdetails

Produktname

Anti-Bif-1 Antibody

Beschreibung

Goat polyclonal antibody to Bif-1.

Anwendungen

ELISA, WB, IHC, Flow Cytometry

Verdünnungen

ELISA: 1:32,000, WB: 0.1-0.3µg/ml, IHC: 2.5µg/ml, Flow Cytometry: 10µg/ml

Reaktivität

Human, Mouse, Rat

Immunogen

Synthetic peptide corresponding to the C terminal region of human Bif-1.

Sequenz

C-QKGKVPITYLELLN

Wirt

Goat

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Konzentration

500 µg/ml

Molekulargewicht

40 kDa

Geschätztes MW

40.8kDa

Produktform

Liquid

Formulierung

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

Bax interacting factor 1, Bax-interacting factor 1, Bif, Bif 1, CGI 61, dJ612B15.2, Endophilin B1, Endophilin-B1, PPP1R70, Protein phosphatase 1 regulatory subunit 70, SH3 containing protein SH3GLB1, SH3 domain GRB2 like endophilin B1, SH3 domain-containing GRB2-like protein B1, Sh3glb1, SH3p13, SHLB1_HUMAN

Isotyp-Kontrollantikörper

Goat IgG (A121671)

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (4)

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Figure 1: Western Blot - Anti-Bif-1 Antibody (A83782)

Bif-1 expression in HEK293 (A) and A431 (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Bif-1 Antibody (A83782) at 0.1µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-Bif-1 Antibody (A83782)

Bif-1 expression in NIH3T3 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Bif-1 Antibody (A83782) at 0.1µg/ml and detected by chemiluminescence.

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Figure 3: Flow Cytometry - Anti-Bif-1 Antibody (A83782)

Bif-1 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Bif-1 Antibody (A83782) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Figure 4: Immunohistochemistry - Anti-Bif-1 Antibody (A83782)

Bif-1 expression in Human Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Bif-1 Antibody (A83782) at 2.5µg/ml and revealed with alkaline phosphatase (AP).