Anti-beta Synuclein Antibody (A270581)

Immunofluorescent analysis of rat cerebellum section stained using Anti-beta Synuclein Antibody (A270581), at a dilution of 1:1,000 in red. The tissue was co-stained using Anti-Parvalbumin Antibody (A85316), at a dilution of 1:5,000, in green. Nuclear DNA is visualised in blue using Hoechst staining. Following transcardial perfusion of the rat with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained using the above antibodies. The Anti-beta Synuclein Antibody (A270581) detects protein concentrated in synaptic regions, and the Anti-Parvalbumin Antibody (A85316) labels the perikarya and dendrites of Purkinje cells, and interneurons in the molecular layer of the cerebellum.

Western blot analysis of different tissue lysates using Anti-beta Synuclein Antibody (A270581), at a dilution of 1:1,000, in green. The lanes contain samples of: [1] Protein standards, in red, [2] mouse cerebellum [3] mouse hippocampus, [4] rat cerebellum, [5] rat hippocampus, and [6] cow cerebellum. The strong band at about 17 kDa corresponds to the beta Synuclein protein.