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Anti-beta Galactosidase Antikörper (A257)

Dieses Produkt ist identisch mit Abcam (ab221199). Entdecken Sie mehr für weniger.

Western blot analysis of beta galactosidase in E. coli crude extract. Immunoprecipitation of beta galactosidase from E. coli crude extract. Immunofluorescence staining of beta galactosidase expressed in HEK293A cells. HEK293A cells were transfected with beta-Gal cDNA, fixed with 4% paraformaldehyde 24 hrs later, permeabilized with methanol, and immunostained with Anti-beta Galactosidase Antibody (1:500) and Anti-Rabbit IgG (Alexa 555) (1/500). Chromosomal DNA was stained with Hoechst 33342. Scale bar, 50 mm. Note that the antibody only reacts with transfected cells. Titration of antibody reactivity of Anti-beta Galactosidase Antibody by ELISA. Plate was coated with 100ng of beta galactosidase per well and 100µl of the antiserum at the indicated dilution was added to each well and incubated. After washing, Goat Anti-Rabbit IgG (HRP) was added as secondary antibody. Color was developed with TMB as substrate.
Empfohlen
für "beta galactosidase Antikörper"
$370

Polyklonal Kaninchen Antikörper gegen beta Galactosidase für WB, IP, ICC/IF und ELISA.

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Versandkosten
$40
Vorlaufzeit
Lieferung in 4-6 Werktagen

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Produktname
Anti-beta Galactosidase Antibody
Beschreibung
Rabbit polyclonal antibody to beta Galactosidase.
Anwendungen
WB, IP, ICC/IF, ELISA
Verdünnungen
WB: 1:1,000-1:2,000, IP: 1:200-1:500, ICC/IF: 1:200-1:500, ELISA: 1:2,000-1:3,000
Reaktivität
Escherichia coli beta Galactosidase and beta Gal Tagged proteins.
Immunogen
Full length E. coli beta Galactosidase.
Wirt
Rabbit
Klonalität
Polyclonal
Isotyp
IgG
Konjugat

Unconjugated

Reinigung
Protein A chromatography.
Konzentration
2 mg/ml
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline with 50% Glycerol.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
Acid beta galactosidase, Beta D galactosidase, Beta gal, Beta galactosidase 1, EBP, ECK0341, ELNR1, Galactosidase beta 1, GLB1, JW0335, Lactase, lacZ, MPS4B
Isotyp-Kontrollantikörper
Kompatible Sekundärantikörper
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Western blot analysis of beta galactosidase in E. coli crude extract.Bild Vergrößern
Figure 1: Anti-beta Galactosidase Antibody (60-060)
Anti-beta Galactosidase Antibody
Immunoprecipitation of beta galactosidase from E. coli crude extract.Bild Vergrößern
Figure 2: Immunoprecipitation - Anti-beta Galactosidase Antibody (60-060)
Anti-beta Galactosidase Antibody
Immunofluorescence staining of beta galactosidase expressed in HEK293A cells. HEK293A cells were transfected with beta-Gal cDNA, fixed with 4% paraformaldehyde 24 hrs later, permeabilized with methanol, and immunostained with Anti-beta Galactosidase Antibody (1:500) and Anti-Rabbit IgG (Alexa 555) (1/500). Chromosomal DNA was stained with Hoechst 33342. Scale bar, 50 mm. Note that the antibody only reacts with transfected cells.Bild Vergrößern
Figure 3: Immunofluorescence - Anti-beta Galactosidase Antibody (60-060)
Anti-beta Galactosidase Antibody
Titration of antibody reactivity of Anti-beta Galactosidase Antibody by ELISA. Plate was coated with 100ng of beta galactosidase per well and 100µl of the antiserum at the indicated dilution was added to each well and incubated. After washing, Goat Anti-Rabbit IgG (HRP) was added as secondary antibody. Color was developed with TMB as substrate.Bild Vergrößern
Figure 4: Anti-beta Galactosidase Antibody (60-060)
Anti-beta Galactosidase Antibody

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