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Anti-Beclin 1 (phospho Ser90) Antibody (A307543)

Western Blot - Anti-Beclin 1 (phospho Ser90) Antibody (A307543) - Antibodies.com
$485
Product Datasheet

Rabbit polyclonal antibody to Beclin 1 (phospho Ser90) for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Beclin 1 (phospho Ser90) Antibody
Description
Rabbit polyclonal antibody to Beclin 1 (phospho Ser90).
Applications
WB
Dilutions
WB: 1:100-1:500
Reactivity
Human, Mouse
Immunogen
A synthetic phosphorylated peptide around S90 of human Beclin 1 (NP_003757.1).
Sequence
MMSTE
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Beclin-1, BECN1, Coiled-coil myosin-like BCL2-interacting protein, GT197, Protein GT197
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-Beclin 1 (phospho Ser90) Antibody (A307543) - Antibodies.comEnlarge Image

Western Blot - Anti-Beclin 1 (phospho Ser90) Antibody (A307543)

Western blot analysis of extracts of various cell lines, using Anti-Beclin 1 (phospho Ser90) Antibody (A307543) at 1:500 dilution. HeLa and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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