Rekombinant Anti-BCMA Antikörper [DM4] (A318679)

Name: Anti-BCMA Antibody [DM4] - BSA and Azide free
Brand: Antibodies.com
SKU: A318679
Price: 665.0 USD
Availability: InStock

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$665

Monoklonal [DM4] Kaninchen Antikörper gegen BCMA für ELISA, Flow Cytometry, IF und IP.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 7-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Stichworte:

Rekombinant Antikörper

Produktdetails

Produktname

Anti-BCMA Antibody [DM4] - BSA and Azide free

Beschreibung

Recombinant rabbit monoclonal [DM4] antibody to BCMA.

Anwendungen

ELISA, Flow Cytometry, IF, IP

Verdünnungen

Flow Cytometry: 1:100, IP: 1:30

Reaktivität

Human

Wirt

Rabbit

Klonalität

Monoclonal

Klon

DM4

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity chromatography.

Konzentration

Reconstitution dependent.

Produktform

Lyophilized

Rekonstitution

Reconstitute with distilled sterile water.

Formulierung

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.

Lagerung

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

Allgemeine Hinweise

Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.

Synonyme

B cell maturation antigen, B cell maturation factor, B cell maturation protein, B-cell maturation protein, BCM, CD269, CD269 antigen, TNFRSF17, TNR17_HUMAN, Tumor necrosis factor receptor superfamily member 17

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (8)

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Figure 1: Flow Cytometry - Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679)

A. Flow cytometry analysis with Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679) on K562-BCMA (Red histogram) (K562 cells stably transduced by human BCMA full length gene) and K562 (Negative control cell line) (Blue histogram). B. Flow cytometry data of serially titrated Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

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Figure 2: Flow Cytometry - Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679)

A. Flow cytometry analysis with Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679) on NCI-H929 cells (Red histogram) or rabbit control antibody on NCI-H929 cells (Blue histogram). B. Flow cytometry data of serially titrated Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679) on NCI-H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

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Figure 3: Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679)

Phylogenetic analysis of different Anti-BCMA Monoclonal Antibody clones. A) Heavy chain and B) Light chain.

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Figure 4: Flow Cytometry - Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679)

Affinity ranking of different Rabbit Anti-BCMA Monoclonal Antibody clones by titration of different concentrations onto K562-BCMA (A) or NCI-H929 cells (B) at 4°C. Bound Rabbit IgG was detected in flow cytometry analysis. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

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Figure 5: Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679)

ELISA plates were pre-coated with Recombinant Human BCMA Protein (Fc Chimera 6xHis Tag) (A318407), followed by pre-blocking with Human Anti-C11D5.3 Antibody (Grey bar) or Rabbit control IgG (Black bar), and then different Rabbit Antibodies were added to check the competitive inhibition of Human Anti-C11D5.3 Antibody. The clone DM3 exhibits the strongest inhibition (Red bar). This data indicated that DM3 bind to the same epitope as bb2121.

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Figure 6: Immunoprecipitation - Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679)

Immunoprecipitation analysis. Cellular overexpression lysates of HEK293F cells transfected with FLAG tagged human BCMA full length gene were pre-incubated with 6 different Rabbit Antibody clones and negative control IgG. The immunocomplexes were further pulled down by Protein A beads, fractionated, and blotted with Mouse Anti-FLAG Monoclonal Antibody.

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Figure 7: Western Blot - Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679)

Western Blot analysis of BCMA protein using Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679) at 1:1000 dilution. Secondary Antibody: Goat Anti-Rabbit IgG (H+L) (HRP) at 1:5000 dilution. 1. K562 cell lysate (negative control). 2. K562 cell lysate with overexpressed human BCMA proteins. 3. MM.1S cell lysate (native BCMA protein).

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Figure 8: Flow Cytometry - Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679)

Flow cytometry analysis of antigen binding of Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679). (A) Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679) does not bind to Jurkat cells that do not express BCMA. (B) A clear peak shift of Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679) was seen compared to the control when incubated with BCMA-expressing MM.1S cells, indicating strong binding of Anti-BCMA Antibody [DM4] - BSA and Azide free (A318679) to BCMA. Antibodies were incubated at 2 µg/ml. .

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