Anti-B7H4 Antibody (A84379)

B7H4 expression in HEK293 iso 1 + iso 2 cell lysate (A) + peptide (B), Human Adrenal Gland iso 1 (C), Human Adrenal Gland iso 2 (D), and Human pancreas iso 1 (E) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-B7H4 Antibody (A84379) at 1µg/ml (A-D) or 0.1µg/ml (E) and detected by chemiluminescence.

B7H4 expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-B7H4 Antibody (A84379) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and plasma membrane/cell junction staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

B7H4 expression in MCF7 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-B7H4 Antibody (A84379) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.