Anti-ATP7B Antikörper [S62-29] (A304969)

Name: Anti-ATP7B Antibody [S62-29]
Brand: Antibodies.com
SKU: A304969
Price: 500.0 USD
Availability: InStock

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$500

Monoklonal [S62-29] Maus Antikörper gegen ATP7B für WB, IHC, ICC/IF und IP.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Produktdetails

Produktname

Anti-ATP7B Antibody [S62-29]

Beschreibung

Mouse monoclonal [S62-29] antibody to ATP7B.

Spezifität

Band detected in rat brain membrane preparations.

Anwendungen

WB, IHC, ICC/IF, IP

Verdünnungen

WB: 1:1,000, ICC/IF: 1:100

Reaktivität

Human, Mouse, Rat

Immunogen

Synthetic peptide amino acids 3-21 (cytoplasmic N-terminus) of human Copper-transporting ATPase2.

Wirt

Mouse

Klonalität

Monoclonal

Klon

S62-29

Isotyp

IgG1

Konjugat

Unconjugated

Reinigung

Protein G purification.

Konzentration

1 mg/ml

Molekulargewicht

~160 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

ATP7B_HUMAN, ATPase, Cu(2+) transporting, beta polypeptide, ATPase, Cu++ transporting, beta polypeptide, Copper pump 2, Copper transporting ATPase 2, PWD, Toxic milk, tx, WC1, WD, Wilson disease associated protein, Wilson disease-associated protein, WND, WND/140 kDa

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Immunofluorescence - Anti-ATP7B Antibody [S62-29] (A304969)

Immunocytochemistry/Immunofluorescence analysis of mouse NIH 3T3, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ATP7B Antibody [S62-29] (A304969), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Copper Transporting ATPase 2 Antibody. (D) Composite.

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Figure 2: Western Blot - Anti-ATP7B Antibody [S62-29] (A304969)

Western blot analysis of rat brain membrane showing detection of ~160 kDa Copper Transporting ATPase 2 protein using Anti-ATP7B Antibody [S62-29] (A304969) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat brain membrane cell lysate. Load: 20 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:100 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~160 kDa.

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Figure 3: Immunofluorescence - Anti-ATP7B Antibody [S62-29] (A304969)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-ATP7B Antibody [S62-29] (A304969), at 1:100 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Copper Transporting ATPase 2 Antibody (D) Composite.