Figure 1: Western Blot - Anti-ATP13A1 Antibody (A84451)
ATP13A1 expression in Human Kidney lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-ATP13A1 Antibody (A84451) at 0.3µg/ml and detected by chemiluminescence.
ATP13A1 expression in Human Thyroid analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-ATP13A1 Antibody (A84451) at 5µg/ml and revealed with alkaline phosphatase (AP).