This antibody recognises human ATG4A (Autophagy-related protein 4 homolog A), otherwise known as APG4A/Cysteine protease ATG4A, a cytosolic cysteine protease and member of the peptidase C54 family, predominantly expressed in skeletal muscle and brain, which plays a central role in the cleavage of the C-terminal region of the target proteins GABARAPL2, MAP1LC3 or GABARAP during autophagy.Rabbit anti Human ATG4A antibody (AHP2220) has been shown to be suitable for use in immunoprecipitation using HEK-293T cell lysates.
Applications
IP
Dilutions
IP: 5 - 10ug
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide corresponding to amino acids 100-114 of human ATG4A, conjugated to Keyhole Limpet Haemocyanin. The corresponding sequence differs by two amino acids in mouse and rat.
Sequence
SWEKQKEQPKEYQRI
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti Human ATG4A antibody recognises human ATG4A (Autophagy-related protein 4 homolog A), otherwise known as APG4A/Cysteine protease ATG4A, a cytosolic cysteine protease and member of the peptidase C54 family, predominantly expressed in skeletal muscle and brain, which plays a central role in the cleavage of the C-Terminal region of the target proteins GABARAPL2, MAP1LC3 or GABARAP during autophagy.Rabbit anti Human ATG4A antibody (AHP2220) has been shown to be suitable for use in immunoprecipitation using HEK-293T cell lysates.
