Figure 1: Western Blot - Anti-ASS1 Antibody (A83511)
ASS1 expression in A431 (A) and NIH3T3(B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-ASS1 Antibody (A83511) at 0.3µg/ml (A)or 1µg/ml (B) and detected by chemiluminescence.
Figure 2: Western Blot - Anti-ASS1 Antibody (A83511)
ASS1 expression in Human Kidney (A), Mouse Liver (B), and Rat Kidney (C) lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-ASS1 Antibody (A83511) at 0.01µg/ml (A-B) or 0.03µg/ml (C) and detected by chemiluminescence.
ASS1 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-ASS1 Antibody (A83511) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
ASS1 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-ASS1 Antibody (A83511) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
ASS1 expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-ASS1 Antibody (A83511) at 2.5µg/ml and revealed with alkaline phosphatase (AP).
ASS1 expression in Human Liver analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-ASS1 Antibody (A83511) at 2.5µg/ml and revealed with alkaline phosphatase (AP).
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