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Anti-APOLD1 Antibody (A326217)

Western Blot - Anti-APOLD1 Antibody (A326217) - Antibodies.com Flow Cytometry - Anti-APOLD1 Antibody (A326217) - Antibodies.com
$465
Product Datasheet

Goat polyclonal antibody to APOLD1 for ELISA, WB and Flow Cytometry.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-APOLD1 Antibody
Description
Goat polyclonal antibody to APOLD1.
Applications
ELISA, WB, Flow Cytometry
Dilutions
ELISA: 1:2,000, WB: 0.5-2µg/ml, Flow Cytometry: 10µg/ml
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C terminal region of human APOLD1.
Sequence
C-KSSRGHDLKISADQ
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
35 kDa
Predicted MW
30.5kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Apolipoprotein L domain-containing protein 1, Vascular early response gene protein, VERGE
Isotype Controls
Suitable Secondaries
See all Anti-Goat IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-APOLD1 Antibody (A326217) - Antibodies.comEnlarge Image

Western Blot - Anti-APOLD1 Antibody (A326217)

APOLD1 expression in A431 (A), Jurkat (B), and U251 (C) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-APOLD1 Antibody (A326217) at 2µg/ml and detected by chemiluminescence.
Flow Cytometry - Anti-APOLD1 Antibody (A326217) - Antibodies.comEnlarge Image

Flow Cytometry - Anti-APOLD1 Antibody (A326217)

APOLD1 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-APOLD1 Antibody (A326217) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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