Independent Antibody Comparison

A comparison of identical primary antibodies from Abcam, Antibodies.com, Novus Biologicals, Sigma Aldrich, and Thermo Fisher.

An independent comparison of identical Anti-AIF1 Antibodies, Anti-GFAP Antibodies, and Anti-Vimentin Antibodies, that are commercially available from Abcam, Antibodies.com, Novus Biologicals, Sigma Aldrich, and Thermo Fisher. All of the antibodies compared are identical and originate from the same primary manufacturers. The antibody testing was performed by an independent laboratory and the slight differences in performance between some of the antibodies is likely due to different lots being tested and / or the different storage histories of each vial.

Anti-GFAP Antibodies

Identical Anti-GFAP Antibodies from Abcam, Antibodies.com, Novus Biologicals, and Thermo Fisher were used for immunofluorescent analysis of paraformaldehyde-fixed U251 cells and western blot analysis of rat brain lysate.

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Supplier	Product Code	Lot	Size	List Price
Abcam	ab7260	GR3287463-4	50µl	$675
Antibodies.com	A85419	X218	50µl	$380
Novus Biologicals	NB300-141	179-031817	50µl	$429
Thermo Fisher	PA3-16727	UJ2864104	50µl	$483

Anti-AIF1 Antibodies

Identical Anti-AIF1 Antibodies from Abcam, Antibodies.com, Novus Biologicals, and Sigma Aldrich were used for immunofluorescent analysis of paraformaldehyde-fixed U937 cells and western blot analysis of mouse brain lysate.

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Supplier	Product Code	Lot	Size	List Price
Abcam	ab5076	GR3322398-1	100µg	$680
Antibodies.com	A82670	X86	100µg	$455
Novus Biologicals	NB100-1028	S7C7G2P41 E131219	100µg	$479
Sigma Aldrich	SAB2500041	5419S7G2P25	100µg	$545

Anti-Vimentin Antibodies

Identical Anti-Vimentin Antibodies [VI-10] from Abcam, Antibodies.com, Novus Biologicals, and Thermo Fisher were used for immunofluorescent analysis of paraformaldehyde-fixed K562 cells and western blot analysis of HeLa cell lysate, HEK293 cell lysate, and Jurkat cell lysate.

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Supplier	Product Code	Lot	Size	List Price
Abcam	ab20346	GR3230520-18	100µg	$630
Antibodies.com	A86652	X84	100µg	$345
Novus Biologicals	NB500-563	530982	100µg	$349
Thermo Fisher	MA1-10459	530982	100µg	$400

* It is interesting to note the identical lot numbers above from Novus Biologicals and Thermo Fisher; rather than reassigning their own lot numbers they have reused the lot number from the primary manufacturer. All online list prices taken in January 2023.