Figure 1: Western Blot - Anti-ANP32B Antibody (A83503)
ANP32B expression in Human Tonsil (A) and Rat (B), and Pig (C) Spleen lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-ANP32B Antibody (A83503) at 0.1µg/ml and detected by chemiluminescence.
Figure 2: Western Blot - Anti-ANP32B Antibody (A83503)
ANP32B expression in NIH3T3 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-ANP32B Antibody (A83503) at 0.1µg/ml and detected by chemiluminescence.
ANP32B expression in Human Breast analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-ANP32B Antibody (A83503) at 3.8µg/ml and revealed with alkaline phosphatase (AP).