Anillin expression in Human Kidney analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in Tris/EDTA buffer, pH 9. Staining was performed with Anti-Anillin Antibody (A83964) at 10µg/ml and revealed with horseradish peroxidase (HRP).
Anillin expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-Anillin Antibody (A83964) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Nuclear staining shown and actin filaments were stained with phalloidin (red). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Anillin expression in MCF7 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Anillin Antibody (A83964) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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