Mouse monoclonal [ACE/3764] antibody to Angiotensin Converting Enzyme 1.
Specificity
This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Two most abundant alternatively spliced variants of this gene encode two isozymes - the somatic form and the testicular form that are equally active. Multiple additional alternatively spliced variants have been identified but their full length nature has not been determined.
Applications
ELISA, IHC-P
Dilutions
IHC-P: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant full-length human Angiotensin Converting Enzyme 1.
Host
Mouse
Clonality
Monoclonal
Clone ID
ACE/3764
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
150 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-Angiotensin Converting Enzyme 1 Antibody [ACE/3764] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma using Anti-Angiotensin Converting Enzyme 1 Antibody [ACE/3764] at 2µg/ml in PBS for 30 minutes at room temperature.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney using Anti-Angiotensin Converting Enzyme 1 Antibody [ACE/3764] at 2µg/ml in PBS for 30 minutes at room temperature.
Analysis of protein array containing more than 19,000 full-length human proteins using Anti-Angiotensin Converting Enzyme 1 Antibody [ACE/3764]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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