This antibody recognises generic epitopes common to many amyloid fibrils and fibrillar oligomers, but not prefibrillar oligomers or natively folded proteins. Expected to detect in Mouse and Rat based on species homology.
Applications
WB, IHC, ICC/IF, IP, ELISA, Dot
Dilutions
WB: 1:1,000, IHC: 1:100
Reactivity
Human
Immunogen
Fibrils prepared from human amyloid beta 42 peptide.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Protein A purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Dot Blot - Anti-Amyloid Fibrils Antibody (A304905)
Dot blot analysis of human cell lysates using Anti-Amyloid Fibrils Antibody (A304905) at 1:500 and 1:5000. Beta Amyloid HEPES-NaCl aggregation, showing 1:500 (L) and 1:5,000 (R) time lapse Dot blot.
Immunohistochemistry analysis of human alzheimer. The Primary Antibody used was Anti-Amyloid Fibrils Antibody (A304905) at 1:100. Showing no Amyloid Precursor Protein (APP) cross-reactivity (L), but when conducted with monoclonal 6E10 (R) shows considerable APP cross-reactivity.
Immunohistochemistry analysis of human alzheimer. The Primary Antibody used was Anti-Amyloid Fibrils Antibody (A304905) at 1:100. Extensive OC labeling was observed in the hippocampus(A), subiculum (B) and frontal cortex (C) in Alzheimer disease. A higher magnification image illustrates that OC positive deposits were dense and consisted of fine fibrillar material (D).
Western Blot - Anti-Amyloid Fibrils Antibody (A304905)
Western blot analysis of human Abeta42 fibrils and prefibrillar oligomers showing detection of Amyloid Fibrils (OC) protein using Anti-Amyloid Fibrils Antibody (A304905) at 1:1,000.
Immunohistochemistry analysis of human alzheimer, fixed in formalin. The Primary Antibody used was Anti-Amyloid Fibrils Antibody (A304905). (C) Composite.
