Anti-AMPK alpha 1 Antibody (A90860)

Name: Anti-AMPK alpha 1 Antibody
Brand: Antibodies.com
SKU: A90860

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This product is discontinued

Anti-AMPK alpha 1 Antibody (A90860) has been discontinued and is no longer available.

View all AMPK alpha 1 Antibodies.

Specifications

Name

Anti-AMPK alpha 1 Antibody

Description

Rabbit polyclonal antibody to AMPK alpha 1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 515-574 of human AMPKa1 (NP_006242.5).

Sequence

YRSCQRSDSDAEAQGKSSEVSLTSSVTSLDSSPVDLTPRPGSHTIEFFEMCANLIKILAQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

64 kDa / 65 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

5'-AMP-activated protein kinase catalytic subunit alpha-1, ACACA kinase, Acetyl-CoA carboxylase kinase, AMPK subunit alpha-1, AMPK1, EC:2.7.11.26, EC:2.7.11.31, HMGCR kinase, Hydroxymethylglutaryl-CoA reductase kinase, PRKAA1, Tau-protein kinase PRKAA1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-AMPK alpha 1 Antibody (A90860)

Western blot analysis of extracts of various cell lines, using Anti-AMPK alpha 1 Antibody (A90860) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunohistochemistry - Anti-AMPK alpha 1 Antibody (A90860)

Immunohistochemistry analysis of paraffin-embedded human smooth muscle using Anti-AMPK alpha 1 Antibody (A90860) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-AMPK alpha 1 Antibody (A90860)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-AMPK alpha 1 Antibody (A90860) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.