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Anti-Ampicillin Aptamer [AMP17] (Biotin) (A320951)

Versandkosten
Vorlaufzeit
Lieferung in 30-45 Werktagen
Telefon
+1 (314) 370-6046
Mo bis Frei, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-Ampicillin Aptamer [AMP17] (Biotin)
Description
Synthetic DNA aptamer to Ampicillin [AMP17] (Biotin).
Specificity
Did not bind to related antibiotics amoxicillin and benzylpenicillin, or unrelated antibiotics chloramphenicol, tetracycline, sulfadimethoxine, streptomycin, and kanamycin.
Conjugate

Biotin

Aptamer Resuspension
Resuspend to 100µM with 10mM Tris-HCl, pH 7.5, and 0.1mM EDTA.
Apatamer Refolding Conditions
Denatured at 90°C for 3 min and then cooled at 4°C for 1 hour.
Apatmer Binding Conditions
Binding Buffer: 20mM Tris-HCl Buffer, pH 8, with 50mM Sodium Chloride, 5mM Potassium Chloride, and 5mM Magnesium Chloride. Binding Temperature: 25°C.
Sequence
5'-dGpdCpdGpdGpdGpdCpdGpdGpdTpdTpdGpdTpdApdTpdApdGpdCpdGpdGp-3'
Aptamer Length
19 nt
Aptamer Molecular Weight (Da)
5,940.9 Da
Aptamer Affinity
Affinity (Kd) = 13.4 nM
Extinction Coefficient
183800 M-1cm-1
Purification
HPLC
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from nuclease-free water (no residual salts).
Storage
Shipped at 4°C. Lyophilized: Store at -20°C. Reconstituted: Store short term at 4°C (i.e. use within days) or aliquot and store at -20°C (medium term: i.e. use within weeks) or -80°C long term. Avoid freeze/thaw cycles.
Product Notes
Anti-Ampicillin Aptamer [AMP17] (Biotin) (A320951) is a replica of the aptamer described in PMID: 22222912.
General Notes
Aptamers have not been validated by Antibodies.com and they need to be tested/evaluated under identical conditions to which they were reported in the literature including, but not limited to, temperature and matrix/buffer conditions. All aptamers are manufactured on request and have a lead time of 30-45 business days.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Oben