Anti-AMBRA1 Antikörper (A305140)

Name: Anti-AMBRA1 Antibody
Brand: Antibodies.com
SKU: A305140
Price: 500.0 USD
Availability: InStock

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$500

Polyklonal Kaninchen Antikörper gegen AMBRA1 für WB und ICC/IF.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Produktdetails

Produktname

Anti-AMBRA1 Antibody

Beschreibung

Rabbit polyclonal antibody to AMBRA1.

Anwendungen

WB, ICC/IF

Verdünnungen

WB: 1:1,000, ICC/IF: 1:100

Reaktivität

Human, Rat

Immunogen

Synthetic peptide of Human AMBRA1 (aa. 200-300).

Wirt

Rabbit

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Peptide affinity purification.

Konzentration

1 mg/ml

Molekulargewicht

~142 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

Activating molecule in beclin 1 regulated autophagy, Activating molecule in BECN1 regulated autophagy protein 1, Activating molecule in BECN1-regulated autophagy protein 1, AMRA1_HUMAN, Autophagy and beclin 1 regulator 1, DCAF3, FLJ20294, Hypothetical protein FLJ20294, Hypothetical protein LOC55626, KIAA1736, MGC33725, WDR94

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (2)

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Figure 1: Immunofluorescence - Anti-AMBRA1 Antibody (A305140)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-AMBRA1 Antibody (A305140), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasmic Vesicle, Autophagosome. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) AMBRA1 Antibody (D) Composite.

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Figure 2: Western Blot - Anti-AMBRA1 Antibody (A305140)

Western blot analysis of rat brain cell lysates showing detection of 142.5 kDa AMBRA1 protein using Anti-AMBRA1 Antibody (A305140) at 1:1,000 for 1 hour at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 142.5 kDa. Other Band(s): 50 kDa.