Beschreibung
Phosphoramidite for the synthesis of oligonucleotides with 5'-terminal alkyne for Click Chemistry. This alkyne amidite has several advantages over 5'-hexynyl phosphoramidite, 5'-butynyl-CEP, and other 5'-terminal alkyne phosphoramidites. First, it is solid compound which is easier to handle and dispense. And due to its structure, it is also more stable in solution, and has a longer shelf life. The diluent for this phosphoramidite is acetonitrile. 5 min coupling time is recommended. Because this amidite does not contain 5'-terminal DMT group, no 5'-deprotection is needed. Oligonucleotides should be deblocked under standard conditions, and purified by PAGE, or ion exchange HPLC. Oligonucleotides with this modification are ideal for use in Click Chemistry.
