AKT3 expression in HepG2 (A), Jurkat (B), Mouse Brain (C), and Rat Brain (D) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-AKT3 Antibody (A83410) at 1µg/ml and detected by chemiluminescence.
AKT3 expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress AKT3 and incubated with Anti-AKT3 Antibody (A83410). Control: Mock-transfected HEK293 cell lysate incubated with Anti-AKT3 Antibody (A83410) (second lane).
AKT3 expression in Human Prostate analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-AKT3 Antibody (A83410) at 5µg/ml and revealed with alkaline phosphatase (AP).
AKT3 expression in Human Heart analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-AKT3 Antibody (A83410) at 5µg/ml and revealed with alkaline phosphatase (AP).
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