This Akt1 (phospho Ser473) Cell Based ELISA Kit allows for the detection of Akt1 (phospho Ser473) and the effects that certain stimulation conditions have on Akt1 (phospho Ser473) expression in different cell lines. Qualitative determination of Akt1 (phospho Ser473) concentration is achieved by an indirect ELISA format. In essence, Akt1 (phospho Ser473) is captured by Anti-Akt1 (phospho Ser473) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-Akt1 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.
Plattform
Microplate
Nachweismethode
Fluorometric
Detektionsbereich
> 5000 Cells
Reaktivität
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Lagerung
Store at + 4°C. Stable for 6 months.
Synonyms
AKT, AKT 1, AKT1_HUMAN, MGC99656, PKB, PKB-ALPHA, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, RAC Alpha, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Figure 4: Western Blot - Akt1 (phospho Ser473) Cell Based ELISA Kit (FLUO-CBP1490)
The mouse monoclonal antibody to GAPDH is used as a positive control in Akt1 (phospho Ser473) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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