Anti-AKT1 (phospho Ser473) Antikörper [ARC0169] (A80471)

Name: Anti-AKT1 (phospho Ser473) Antibody [ARC0169]
Brand: Antibodies.com
SKU: A80471
Price: 540.0 USD
Availability: InStock

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$540

Monoklonal [ARC0169] Kaninchen Antikörper gegen AKT1 (phospho Ser473) für WB und IHC.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Produktdetails

Produktname

Anti-AKT1 (phospho Ser473) Antibody [ARC0169]

Beschreibung

Rabbit monoclonal [ARC0169] antibody to AKT1 (phospho Ser473).

Anwendungen

WB, IHC

Verdünnungen

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S473 of human Akt1 (P31749).

Sequenz

QFSYS

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC0169

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

60 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

AKT, AKT 1, AKT1_HUMAN, MGC99656, PKB, PKB-ALPHA, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, RAC Alpha, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (6)

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Figure 1: Western Blot - Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471)

Western blot analysis of extracts of various cell lines, using Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471) at 1:1,000 dilution. Both Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471) at 1:1,000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Immunohistochemistry - Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-AKT1 (phospho Ser473) Antibody [ARC0169] (A80471) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.