Anti-AKT1 Antikörper [ARC51585] (A309277)

Name: Anti-AKT1 Antibody [ARC51585]
Brand: Antibodies.com
SKU: A309277
Price: 470.0 USD
Availability: InStock

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$470

Monoklonal [ARC51585] Kaninchen Antikörper gegen AKT1 für WB, IHC und IP.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

100% Qualitätsgarantie

Preis-Match-Garantie

Stichworte:

Knockout-validierter Antikörper

Produktdetails

Produktname

Anti-AKT1 Antibody [ARC51585]

Beschreibung

Rabbit monoclonal [ARC51585] antibody to AKT1.

Anwendungen

WB, IHC, IP

Verdünnungen

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reaktivität

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human AKT1.

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC51585

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

AKT, AKT 1, AKT1_HUMAN, MGC99656, PKB, PKB-ALPHA, PRKBA, Protein kinase B, Protein Kinase B Alpha, Proto-oncogene c-Akt, RAC, RAC Alpha, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (5)

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Figure 1: Western Blot - Anti-AKT1 Antibody [ARC51585] (A309277)

Western blot analysis of extracts of various cell lines, using Anti-AKT1 Antibody [ARC51585] (A309277) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-AKT1 Antibody [ARC51585] (A309277)

Western blot analysis of extracts from wild type(WT) and AKT1 knockout (KO) HeLa cells, using Anti-AKT1 Antibody [ARC51585] (A309277) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 3: Immunohistochemistry - Anti-AKT1 Antibody [ARC51585] (A309277)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-AKT1 Antibody [ARC51585] (A309277) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-AKT1 Antibody [ARC51585] (A309277)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-AKT1 Antibody [ARC51585] (A309277) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.