Anti-AKAP5 Antibody (A15259)

Name: Anti-AKAP5 Antibody
Brand: Antibodies.com
SKU: A15259
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to AKAP5 for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-AKAP5 Antibody

Description

Rabbit polyclonal antibody to AKAP5.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:100-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human AKAP5 (NP_004848.3).

Sequence

METTISEIHVENKDEKRSAEGSPGAERQKEKASMLCFKRRKKAAKALKPKAGSEAADVARKCPQEAGASDQPEPTRGAWASLKRLVTRRKRSESSKQQKPLEGEMQPAINAEDADLSKKKAKSRLKIPCIKFPRGPKRSNHSKIIEDSDCSIKVQEEAEILDIQTQTPLNDQATKAKSTQDLSEGISRKDGDEVCESNVSNSTTSGEKVISVELGLDNGHSAIQTGTLILEEIETIKEKQDVQPQQASPLETSETDHQQPVLSDVPPLPA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

47 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

A-kinase anchor protein 5, A-kinase anchor protein 79 kDa, AKAP 79, AKAP-5, AKAP79, cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein, H21

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-AKAP5 Antibody (A15259)

Western blot analysis of extracts of various cell lines, using Anti-AKAP5 Antibody (A15259) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunohistochemistry - Anti-AKAP5 Antibody (A15259)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-AKAP5 Antibody (A15259) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-AKAP5 Antibody (A15259)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-AKAP5 Antibody (A15259) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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