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AKAP13 Cell Based Kit Elisa (A103656)

$550
Versandkosten
Vorlaufzeit
Lieferung in 5-8 Werktagen
Telefon
+1 (314) 370-6046
Mo bis Frei, 8am - 4pm AST
Email
orders@antibodies.com
Produktname
AKAP13 Cell Based ELISA Kit
Assay-Typ
Cell-based (qualitative)
Assay-Prinzip
This AKAP13 Cell Based ELISA Kit allows for the detection of AKAP13 and the effects that certain stimulation conditions have on AKAP13 expression in different cell lines. Qualitative determination of AKAP13 concentration is achieved by an indirect ELISA format. In essence, the AKAP13 is captured by Anti-AKAP13 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this AKAP13 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plattform
Microplate
Nachweismethode
Colorimetric
Probentyp
Adherent cells and suspension cells.
Detektionsbereich
> 5000 Cells
Testdauer
4 hours 30 minutes
Reaktivität
Human
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Lagerung
Store at + 4°C. Stable for 6 months.
Synonyms
A kinase (PRKA) anchor protein 13, A kinase anchor protein 13, A kinase anchoring protein, A-kinase anchor protein 13, AKAP 13, AKAP Lbc, AKAP-13, AKAP-Lbc, AKP13_HUMAN, ARHGEF13, Breast cancer nuclear receptor binding auxiliary protein, Breast cancer nuclear receptor-binding auxiliary protein, BRX, c lbc, FLJ11952, FLJ43341, Guanine nucleotide exchange factor Lbc, HA 3, HA3, Ht 31, Ht31, Human thyroid anchoring protein 31, Human thyroid-anchoring protein 31, LBC, LBC oncogene, Lymphoid blast crisis oncogene, Non oncogenic Rho GTPase specific GTP exchange factor, Non-oncogenic Rho GTPase-specific GTP exchange factor, p47, Protein kinase A anchoring protein 13, Protein kinase A-anchoring protein 13, PROTO LB, PROTO LBC
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
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