Western Blot - Anti-AHA1 Antibody [4H9-D7] (A304911)
Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of Aha1 protein using Anti-AHA1 Antibody [4H9-D7] (A304911) at 1:1,000. (1) HeLa cell lysate (2) recombinant Aha1 cell lysate.
Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-AHA1 Antibody [4H9-D7] (A304911), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) AHA1 Antibody. (D) Composite.
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