Anti-FACL4 Antikörper (A83246)

Name: Anti-FACL4 Antibody
Brand: Antibodies.com
SKU: A83246
Price: 480.0 USD
Availability: InStock

Dieses Produkt ist identisch mit Abcam (ab110007). Entdecken Sie mehr für weniger.

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$480

Polyklonal Ziege Antikörper gegen FACL4 für ELISA, WB, IHC, IF und Flow Cytometry.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 3-5 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Produktdetails

Produktname

Anti-FACL4 Antibody

Beschreibung

Goat polyclonal antibody to FACL4.

Anwendungen

ELISA, WB, IHC, IF, Flow Cytometry

Verdünnungen

ELISA: 1:128,000, WB: 0.1-0.3µg/ml, IHC: 3.75µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reaktivität

Human

Immunogen

Synthetic peptide corresponding to the C terminal region of human FACL4.

Sequenz

C-HYLKDIERMYGGK

Wirt

Goat

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Konzentration

500 µg/ml

Molekulargewicht

80-85 kDa

Geschätztes MW

79.2kDa

Produktform

Liquid

Formulierung

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

ACS 4, ACS4, ACSL 4, Acsl4, ACSL4_HUMAN, acyl CoA synthetase 4, Acyl CoA synthetase long chain family member 4, FACL 4, Fatty acid Coenzyme A ligase, fatty acid Coenzyme A ligase long-chain 4, LACS 4, LACS4, Lignoceroyl CoA synthase, Long chain 4, long chain acyl CoA synthetase 4, long chain fatty acid CoA ligase 4, long chain fatty acid Coenzyme A ligase 4, Long-chain acyl-CoA synthetase 4, Long-chain-fatty-acid--CoA ligase 4, MRX63, MRX68

Isotyp-Kontrollantikörper

Goat IgG (A121671)

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (5)

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Figure 1: Western Blot - Anti-FACL4 Antibody (A83246)

FACL4 expression in HepG2 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-FACL4 Antibody (A83246) at 0.1µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-FACL4 Antibody (A83246)

FACL4 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-FACL4 Antibody (A83246) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. ER and Golgi apparatus staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Flow Cytometry - Anti-FACL4 Antibody (A83246)

FACL4 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-FACL4 Antibody (A83246) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Figure 4: Immunohistochemistry - Anti-FACL4 Antibody (A83246)

FACL4 expression in Human Small Intestine analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-FACL4 Antibody (A83246) at 3.75µg/ml and revealed with alkaline phosphatase (AP).