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Anti-ACSL1 Antibody (A91192)

Western Blot - Anti-ACSL1 Antibody (A91192) - Antibodies.com Immunofluorescence - Anti-ACSL1 Antibody (A91192) - Antibodies.com Immunofluorescence - Anti-ACSL1 Antibody (A91192) - Antibodies.com Western Blot - Anti-ACSL1 Antibody (A91192) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to ACSL1 for WB, ICC/IF and IP.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-ACSL1 Antibody
Description
Rabbit polyclonal antibody to ACSL1.
Applications
WB, ICC/IF, IP
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 46-145 of human ACSL1 (NP_001986.2).
Sequence
TRPKPLKPPCDLSMQSVEVAGSGGARRSALLDSDEPLVYFYDDVTTLYEGFQRGIQVSNNGPCLGSRKPDQPYEWLSYKQVAELSECIGSALIQKGFKTA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
78 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
ACS1, Acyl-CoA synthetase 1, Arachidonate--CoA ligase, EC:6.2.1.15, FACL1, FACL2, LACS, LACS 1, LACS 2, LACS1, LACS2, Long-chain acyl-CoA synthetase 1, Long-chain acyl-CoA synthetase 2, Long-chain fatty acid-CoA ligase 2, Long-chain-fatty-acid--CoA ligase 1
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-ACSL1 Antibody (A91192) - Antibodies.comEnlarge Image

Western Blot - Anti-ACSL1 Antibody (A91192)

Western blot analysis of extracts of various cell lines, using Anti-ACSL1 Antibody (A91192) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence - Anti-ACSL1 Antibody (A91192) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-ACSL1 Antibody (A91192)

Immunofluorescence analysis of C6 cells using Anti-ACSL1 Antibody (A91192) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-ACSL1 Antibody (A91192) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-ACSL1 Antibody (A91192)

Immunofluorescence analysis of NIH-3T3 cells using Anti-ACSL1 Antibody (A91192) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-ACSL1 Antibody (A91192) - Antibodies.comEnlarge Image

Western Blot - Anti-ACSL1 Antibody (A91192)

Immunoprecipitation analysis of 300µg extracts of Raji cells using 3µg of Anti-ACSL1 Antibody (A91192). This Western blot was performed on the immunoprecipitate using Anti-ACSL1 Antibody (A91192) at a dilution of 1:1000.
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