This Acetylcholinesterase Activity Assay Kit is a quantitative assay kit for measuring Acetylcholinesterase activity in fresh serum, fresh plasma, animal tissues, and nerve cells.
Anwendungen
Functional Studies
Assay-Prinzip
This kit provides a convenient tool for detection of acetylcholinesterase (AchE) activity. The principle is that AchE catalyzes the hydrolysis of acetylcholine to produce choline. Choline reacts with dithiopara-nitrobenzoic acid (DTNB) to produce 5-mercapto-nitrobenzoic acid (TNB). TNB has a maximum absorption peak detected at 412 nm. The enzyme activity of AchE is calculated by measuring the rate of increase in absorption at 412 nm.
Probentyp
Fresh serum, fresh plasma, animal tissues, and nerve cells.
