Assay-Prinzip
Acetylcholine ELISA Kit employs the Competitive Enzyme Immunoassay (ELISA) technique for the quantitative measurement ofuniversalAcetylcholine in serum, plasma, tissue homogenates or other biological fluids. A monoclonal antibody specific for Acetylcholine has been pre-coated onto a 96-well microtiter plate. During the incubation, Acetylcholine in the sample or standard competes with a fixed amount of Biotinylated-Acetylcholine. Following incubation, Biotinylated-Acetylcholine and free Acetylcholine in the sample or standard are washed from the plate. Avidin conjugated to Horseradish Peroxidase (Avidin-HRP) is then added to each well and incubated. TMB substrate solution is then added to each well, and after incubation, the enzyme-substrate reaction is terminated by the addition of sulfuric acid stop solution. The color change is measured spectrophotometrically at a wavelength of 450nm, and the concentration of Acetylcholine in the samples is then determined by comparing the O.D. 450nm of the samples to the standard curve. The O.D. 450nm is inversely proportional to the concentration of Acetylcholine in the sample.
