Anti-ABCC9 Antikörper [S319A-14] (A304816)

Name: Anti-ABCC9 Antibody [S319A-14]
Brand: Antibodies.com
SKU: A304816
Price: 500.0 USD
Availability: InStock

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$500

Monoklonal [S319A-14] Maus Antikörper gegen ABCC9 für WB, IHC und ICC/IF.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Produktdetails

Produktname

Anti-ABCC9 Antibody [S319A-14]

Beschreibung

Mouse monoclonal [S319A-14] antibody to ABCC9.

Anwendungen

WB, IHC, ICC/IF

Verdünnungen

WB: 1:1,000

Reaktivität

Human, Mouse, Rat

Kreuzreaktivität

This antibody does not cross-react with SUR2B.

Immunogen

Fusion protein amino acids 1505-1546 (SSIVDAGLVLVFSEGILVECDTGPNLLQHKNGLFSTLVMTNK, cytoplasmic C-terminus) of mouse SUR2A.

Wirt

Mouse

Klonalität

Monoclonal

Klon

S319A-14

Isotyp

IgG2a

Konjugat

Unconjugated

Reinigung

Protein G purification.

Konzentration

1 mg/ml

Molekulargewicht

~120 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.1% Sodium Azide

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

ABC37, ABCC9_HUMAN, AI414027, AI449286, ATFB12, ATP-binding cassette sub-family C member 9, ATP-binding cassette transporter sub-family C member 9, ATP-binding cassette, sub-family C (CFTR/MRP), member 9, CANTU, CMD1O, FLJ36852, Sulfonylurea receptor 2, Sulfonylurea-binding protein 2, SUR2, SUR2A, SUR2B

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Immunofluorescence - Anti-ABCC9 Antibody [S319A-14] (A304816)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-ABCC9 Antibody [S319A-14] (A304816), at 1:200 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SUR2A Antibody (D) Composite.

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Figure 2: Western Blot - Anti-ABCC9 Antibody [S319A-14] (A304816)

Western blot analysis of rat brain membrane showing detection of ~120 kDa SUR2A protein using Anti-ABCC9 Antibody [S319A-14] (A304816) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: rat brain membrane (10 µg).. Load: 10 µg. Block: 5% milk. The secondary antibody used was Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at room temperature. Color Development: TMB solution for 10 min at room temperature. Predicted/Observed Size: ~120 kDa.

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Figure 3: Immunofluorescence - Anti-ABCC9 Antibody [S319A-14] (A304816)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ABCC9 Antibody [S319A-14] (A304816), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Membrane. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SUR2A Antibody. (D) Composite.