Rabbit polyclonal antibody to 4E-BP1 (Phospho-Thr46)
Specificity
The antibody detects endogenous level of 4E-BP1 only when phosphorylated at Threonine 45.
Applications
WB, IHC
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 46 (S-T-T(p)-P-G) derived from Human 4E-BP1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
18kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
eIF4E-binding protein 1, EIF4EBP1, Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-I, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Western blot analysis of extracts from Hela cells using 4E-BP1 (Phospho-Thr46) Antibody #11223 and the same antibody preincubated with blocking peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Phospho-Thr45) Antibody #11223 (left) or the same antibody preincubated with blocking peptide (right).
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