Anti-DDX3Y Antibody (A80871)

Name: Anti-DDX3Y Antibody
Brand: Antibodies.com
SKU: A80871
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to DDX3Y for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-DDX3Y Antibody

Description

Rabbit polyclonal antibody to DDX3Y.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DDX3Y (NP_001116137.1).

Sequence

MSHVVVKNDPELDQQLANLDLNSEKQSGGASTASKGRYIPPHLRNREASKGFHDKDSSGWSCSKDKDAYSSFGSRDSRGKPGYFSERGSGSRGRFDDRGRSDYDGIGNRERPGFGRFERSGHSRWCDKSVEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGSNCPPHIENFSDIDMGEIIMGNIELTRYTRPTPVQKHAIP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

73 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ATP dependent RNA helicase DDX3Y, ATP-dependent RNA helicase DDX3Y, DBY, DDX3Y_HUMAN, DEAD (Asp-Glu-Ala-Asp) box polypeptide 3 Y linked, DEAD box protein 3, DEAD box protein 3 Y chromosomal, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide Y chromosome, Y-chromosomal

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Western Blot - Anti-DDX3Y Antibody (A80871)

Western blot analysis of extracts of various cell lines, using Anti-DDX3Y Antibody (A80871) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-DDX3Y Antibody (A80871)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-DDX3Y Antibody (A80871) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-DDX3Y Antibody (A80871)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-DDX3Y Antibody (A80871) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-DDX3Y Antibody (A80871)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-DDX3Y Antibody (A80871) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.