Anti-Dcp1a Antibody [ARC1421] (A10006)

Name: Anti-Dcp1a Antibody [ARC1421]
Brand: Antibodies.com
SKU: A10006
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1421] antibody to Dcp1a for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Dcp1a Antibody [ARC1421]

Description

Rabbit monoclonal [ARC1421] antibody to Dcp1a.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 483-582 of human DCP1A (Q9NPI6).

Sequence

PVAGAPLVTATTTAVSSVLLAPSVFQQTVTRSSDLERKASSPSPLTIGTPESQRKPSIILSKSQLQDTLIHLIKNDSSFLSTLHEVYLQVLTKNKDNHNL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1421

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

73 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DCP1 decapping enzyme homolog A, DCP1A_HUMAN, Decapping enzyme hDcp1a, Decapping mRNA 1A, HSA275986, mRNA decapping enzyme 1A, mRNA-decapping enzyme 1A, Nbla00360, Putative protein product of Nbla00360, Smad4 interacting transcriptional co activator, Smad4-interacting transcriptional co-activator, SMAD4IP1, SMIF, Transcription factor SMIF

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Dcp1a Antibody [ARC1421] (A10006)

Western blot analysis of extracts of various cell lines, using Anti-Dcp1a Antibody [ARC1421] (A10006) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-Dcp1a Antibody [ARC1421] (A10006)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Dcp1a Antibody [ARC1421] (A10006) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-Dcp1a Antibody [ARC1421] (A10006)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Dcp1a Antibody [ARC1421] (A10006) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-Dcp1a Antibody [ARC1421] (A10006)

Immunofluorescence analysis of U-2 OS cells using Anti-Dcp1a Antibody [ARC1421] (A10006) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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