Anti-DAZL Antibody (A89480)

Name: Anti-DAZL Antibody
Brand: Antibodies.com
SKU: A89480
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to DAZL for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-DAZL Antibody

Description

Rabbit polyclonal antibody to DAZL.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 201-250 of human DAZL (NP_001342.2).

Sequence

RSYVVPPAYSAVNYHCNEVDPGAEVVPNECSVHEATPPSGNGPQKKSVDR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

38 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DAZ homolog, DAZ like autosomal, DAZ-like autosomal, DAZH, DAZL 1, DAZL1, DAZLA, DAZL_HUMAN, Deleted in azoospermia like, Deleted in azoospermia like 1, Deleted in azoospermia like autosomal, Deleted in azoospermia-like, Deleted in azoospermia-like 1, Germline specific RNA binding protein, MGC26406, Spermatogenesis gene on the Y like autosomal, SPGY like autosomal, SPGY-like-autosomal, SPGYLA, Tpx2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-DAZL Antibody (A89480)

Western blot analysis of extracts of various cell lines, using Anti-DAZL Antibody (A89480) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-DAZL Antibody (A89480)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-DAZL Antibody (A89480) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-DAZL Antibody (A89480)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-DAZL Antibody (A89480) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-DAZL Antibody (A89480)

Immunofluorescence analysis of rat testis using Anti-DAZL Antibody (A89480) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).