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Anti-Cytokeratin 7 Antibody (A92201)

Western Blot - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.com Western Blot - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.com Immunofluorescence - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.com Immunofluorescence - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.com Immunofluorescence - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Cytokeratin 7 for WB, IHC and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Cytokeratin 7 Antibody
Description
Rabbit polyclonal antibody to Cytokeratin 7.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 240-355 of human Cytokeratin 7 (KRT7) (NP_005547.3).
Sequence
ISDTSVVLSMDNSRSLDLDGIIAEVKAQYEEMAKCSRAEAEAWYQTKFETLQAQAGKHGDDLRNTRNEISEMNRAIQRLQAEIDNIKNQRAKLEAAIAEAEERGELALKDARAKQE
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
51 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CK 7, CK-7, CK7, Cytokeratin-7, D15Wsu77e, K2C7, K2C7_HUMAN, K7, Keratin 7, Keratin 7, type II, Keratin type II cytoskeletal 7, Keratin, 55K type II cytoskeletal, Keratin, simple epithelial, Keratin, simple epithelial type I, K7, Keratin, type II cytoskeletal 7, Keratin-7, Krt2-7, KRT7, MGC11625, MGC129731, MGC3625, Sarcolectin, SCL, Type II mesothelial keratin K7, Type-II keratin Kb7
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Cytokeratin 7 Antibody (A92201)
Western blot analysis of extracts of HepG2 cells, using Anti-Cytokeratin 7 Antibody (A92201) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
Western Blot - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Cytokeratin 7 Antibody (A92201)
Western blot analysis of extracts of Rat lung, using Anti-Cytokeratin 7 Antibody (A92201) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-Cytokeratin 7 Antibody (A92201)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-Cytokeratin 7 Antibody (A92201) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Cytokeratin 7 Antibody (A92201) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-Cytokeratin 7 Antibody (A92201)
Immunofluorescence analysis of human lung cancer using Anti-Cytokeratin 7 Antibody (A92201) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-Cytokeratin 7 Antibody (A92201)? Please let us know so that we can list the citation on this page.

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