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Anti-Cytokeratin 18 Antibody [ARC2303] (A305288)

Western Blot - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) - Antibodies.com Immunohistochemistry - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) - Antibodies.com Immunofluorescence - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) - Antibodies.com Immunofluorescence - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) - Antibodies.com Immunofluorescence - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC2303] antibody to Cytokeratin 18 for WB, IHC and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Cytokeratin 18 Antibody [ARC2303]
Description
Rabbit monoclonal [ARC2303] antibody to Cytokeratin 18.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:1,000-1:3,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 250-430 of human Cytokeratin 18 (KRT18) (P05783).
Sequence
ADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC2303
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
48 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Cell proliferation inducing gene 46 protein, Cell proliferation inducing protein 46, Cell proliferation-inducing gene 46 protein, CK 18, CK-18, CK18, CYK 18, CYK18, Cytokeratin endo B, Cytokeratin-18, K 18, K18, K1C18_HUMAN, KA18, Keratin 18, Keratin 18, type I, Keratin D, keratin, type I cytoskeletal 18, Keratin-18, Krt18
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288)
Western blot analysis of extracts of various cell lines, using Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288)
Immunohistochemistry analysis of paraffin-embedded rat liver using Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) - Antibodies.comEnlarge Image
Figure 3: Immunohistochemistry - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288)
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) - Antibodies.comEnlarge Image
Figure 4: Immunohistochemistry - Anti-Cytokeratin 18 Antibody [ARC2303] (A305288)
Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-Cytokeratin 18 Antibody [ARC2303] (A305288) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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