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Anti-Cytokeratin 15 Antibody [ARC0270] (A309189)

Western Blot - Anti-Cytokeratin 15 Antibody [ARC0270] (A309189) - Antibodies.com Immunofluorescence - Anti-Cytokeratin 15 Antibody [ARC0270] (A309189) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC0270] antibody to Cytokeratin 15 for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Cytokeratin 15 Antibody [ARC0270]
Description
Rabbit monoclonal [ARC0270] antibody to Cytokeratin 15.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 357-456 of human Cytokeratin 15 (KRT15) (KRT15) (P19012).
Sequence
YATQLQQIQGLIGGLEAQLSELRCEMEAQNQEYKMLLDIKTRLEQEIATYRSLLEGQDAKMAGIAIREASSGGGGSSSNFHINVEESVDGQVVSSHKREI
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0270
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
49 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AI528832, CK 15, CK-15, CK15, Cytokeratin-15, Cytokeratin15, K15, K1C15_HUMAN, K1CO, Ka15, Keratin, Keratin 15, Keratin 15 basic, Keratin 15 beta, Keratin complex 1 acidic gene 15, Keratin type I cytoskeletal 15, Keratin-15, Keratin15, KRT 15, Krt1-15, KRT15, KRTB, KRTL15, Type I cytoskeletal 15, Type I keratin Ka15
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Cytokeratin 15 Antibody [ARC0270] (A309189) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Cytokeratin 15 Antibody [ARC0270] (A309189)
Western blot analysis of extracts of Cal-27 cells, using Anti-Cytokeratin 15 Antibody [ARC0270] (A309189) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence - Anti-Cytokeratin 15 Antibody [ARC0270] (A309189) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Cytokeratin 15 Antibody [ARC0270] (A309189)
Immunofluorescence analysis of rat skin using Anti-Cytokeratin 15 Antibody [ARC0270] (A309189) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-Cytokeratin 15 Antibody [ARC0270] (A309189)? Please let us know so that we can list the citation on this page.

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