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Anti-Cytokeratin 15 Antibody (A14120)

Western Blot - Anti-Cytokeratin 15 Antibody (A14120) - Antibodies.com Immunofluorescence - Anti-Cytokeratin 15 Antibody (A14120) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Cytokeratin 15 for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Cytokeratin 15 Antibody
Description
Rabbit polyclonal antibody to Cytokeratin 15.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Cytokeratin 15 (KRT15) (P19012).
Sequence
LLSGNEKITMQNLNDRLASYLDKVRALEEANADLEVKIHDWYQKQTPTSPECDYSQYFKTIEELRDKIMATTIDNSRVILEIDNARLAADDFRLKYENELA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
47 - 50 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AI528832, CK 15, CK-15, CK15, Cytokeratin-15, Cytokeratin15, K15, K1C15_HUMAN, K1CO, Ka15, Keratin, Keratin 15, Keratin 15 basic, Keratin 15 beta, Keratin complex 1 acidic gene 15, Keratin type I cytoskeletal 15, Keratin-15, Keratin15, KRT 15, Krt1-15, KRT15, KRTB, KRTL15, Type I cytoskeletal 15, Type I keratin Ka15
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Cytokeratin 15 Antibody (A14120) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Cytokeratin 15 Antibody (A14120)
Western blot analysis of extracts of various cell lines, using Anti-Cytokeratin 15 Antibody (A14120) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.
Immunofluorescence - Anti-Cytokeratin 15 Antibody (A14120) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-Cytokeratin 15 Antibody (A14120)
Immunofluorescence analysis of A431 cells using Anti-Cytokeratin 15 Antibody (A14120) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-Cytokeratin 15 Antibody (A14120)? Please let us know so that we can list the citation on this page.

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