Anti-Cytochrome P450 Reductase Antibody (A16086)

Name: Anti-Cytochrome P450 Reductase Antibody
Brand: Antibodies.com
SKU: A16086
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to Cytochrome P450 Reductase for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Knockout Validated Antibody

Specifications

Name

Anti-Cytochrome P450 Reductase Antibody

Description

Rabbit polyclonal antibody to Cytochrome P450 Reductase.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CYPOR (NP_000932.3).

Sequence

MINMGDSHVDTSSTVSEAVAEEVSLFSMTDMILFSLIVGLLTYWFLFRKKKEEVPEFTKIQTLTSSVRESSFVEKMKKTGRNIIVFYGSQTGTAEEFANRLSKDAHRYGMRGMSADPEEYDLADLSSLPEIDNALVVFCMATYGEGDPTDNAQDFYDWLQETDVDLSGVKFAVFGLGNKTYEHFNAMGKYVDKRLEQLGAQRIFELGLGDDDGNLEEDFITWREQFWPAVCEHFGVEATGEESSIRQYEL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

77 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CPR, CYPOR, Cytochrome p450 oxidoreductase, DKFZp686G04235, FLJ26468, NADPH Cytochrome P450 Reductase, NADPH dependent cytochrome P450 reductase, NADPH--cytochrome P450 reductase, NCPR_HUMAN, P450 (cytochrome) oxidoreductase, P450 Cytochrome Oxidoreductase, P450R, por

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Cytochrome P450 Reductase Antibody (A16086)

Western blot analysis of extracts of various cell lines, using Anti-Cytochrome P450 Reductase Antibody (A16086) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Western Blot - Anti-Cytochrome P450 Reductase Antibody (A16086)

Western blot analysis of extracts from normal (control) and CYPOR knockout (KO) HeLa cells, using Anti-Cytochrome P450 Reductase Antibody (A16086) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunohistochemistry - Anti-Cytochrome P450 Reductase Antibody (A16086)

Immunohistochemistry analysis of paraffin-embedded human esophagus using Anti-Cytochrome P450 Reductase Antibody (A16086) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Cytochrome P450 Reductase Antibody (A16086)

Immunofluorescence analysis of C6 cells using Anti-Cytochrome P450 Reductase Antibody (A16086) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).