Anti-Cytochrome P450 2E1 Antibody (A13920)

Name: Anti-Cytochrome P450 2E1 Antibody
Brand: Antibodies.com
SKU: A13920
Price: 335.0 USD
Availability: InStock

Enlarge Image

$335

Rabbit polyclonal antibody to Cytochrome P450 2E1 for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-Cytochrome P450 2E1 Antibody

Description

Rabbit polyclonal antibody to Cytochrome P450 2E1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 234-493 of human CYP2E1 (NP_000764.1).

Sequence

KVIKNVAEVKEYVSERVKEHHQSLDPNCPRDLTDCLLVEMEKEKHSAERLYTMDGITVTVADLFFAGTETTSTTLRYGLLILMKYPEIEEKLHEEIDRVIGPSRIPAIKDRQEMPYMDAVVHEIQRFITLVPSNLPHEATRDTIFRGYLIPKGTVVVPTLDSVLYDNQEFPDPEKFKPEHFLNENGKFKYSDYFKPFSTGKRVCAGEGLARMELFLLLCAILQHFNLKPLVDPKDIDLSPIHIGFGCIPPRYKLCVIPRS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

4-nitrophenol 2-hydroxylase, CP2E1_HUMAN, CPE1, CYP2E, Cyp2e1, CYPIIE1, Cytochrome P450 isozyme 3A, Cytochrome P450 LM3A, Cytochrome P450, family 2, subfamily e, polypeptide 1, Cytochrome P450, subfamily IIE, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, Cytochrome P450-ALC, Cytochrome P450-J, Cytochrome P450RLM6, EC 1.14.13.-, EC 1.14.13.n7, Ethanol-inducible P450, Flavoprotein-linked monooxygenase, MGC133529, Microsomal monooxygenase, OTTHUMP00000020813, OTTHUMP00000046571, P450 ALC, P450-J, P450C2E, P450J, P450RLM6, Xenobiotic monooxygenase

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

Enlarge Image

Figure 1: Western Blot - Anti-Cytochrome P450 2E1 Antibody (A13920)

Western blot analysis of extracts of Rat liver, using Anti-Cytochrome P450 2E1 Antibody (A13920) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 0.5s.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-Cytochrome P450 2E1 Antibody (A13920)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Cytochrome P450 2E1 Antibody (A13920) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-Cytochrome P450 2E1 Antibody (A13920)

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-Cytochrome P450 2E1 Antibody (A13920) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunofluorescence - Anti-Cytochrome P450 2E1 Antibody (A13920)

Immunofluorescence analysis of mouse liver cells using Anti-Cytochrome P450 2E1 Antibody (A13920) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).