Figure 1: Western Blot - Anti-Cytochrome C Antibody (A88286)
Western blot analysis of extracts of various cell lines, using Anti-Cytochrome C Antibody (A88286) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Figure 2: Immunohistochemistry - Anti-Cytochrome C Antibody (A88286)
Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-Cytochrome C Antibody (A88286) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Figure 3: Immunohistochemistry - Anti-Cytochrome C Antibody (A88286)
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-Cytochrome C Antibody (A88286) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Figure 4: Immunohistochemistry - Anti-Cytochrome C Antibody (A88286)
Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Cytochrome C Antibody (A88286) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Figure 5: Immunohistochemistry - Anti-Cytochrome C Antibody (A88286)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Cytochrome C Antibody (A88286) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Figure 6: Immunofluorescence - Anti-Cytochrome C Antibody (A88286)
Immunofluorescence analysis of HepG2 cells using Anti-Cytochrome C Antibody (A88286) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Figure 7: Immunofluorescence - Anti-Cytochrome C Antibody (A88286)
Immunofluorescence analysis of NIH/3T3 cells using Anti-Cytochrome C Antibody (A88286) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).